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In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 54, Heft 1, S. 72-79
ISSN: 2524-2342
In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 57, Heft 1, S. 41-47
ISSN: 2524-2342
An effective method for the preparation of water-soluble cyanine dye Cy5 using the soluble polymers supported liquid-phase organic synthesis (LPOS) was proposed. Poly (ethylene glycol) methyl ether with a molecular weight of 2000 was used as a polymer substrate, which allowed us to simplify the characterization of products at intermediate stages of synthesis by NMR spectroscopy. This approach makes it easy to obtain the necessary cyanine dyes, which are widely used as fluorescent labels and are popular modifying reagents in biochemistry and medicine.
In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 54, Heft 2, S. 197-203
ISSN: 2524-2342
In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 54, Heft 1, S. 46-57
ISSN: 2524-2342
In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 58, Heft 4, S. 398-406
ISSN: 2524-2342
A synthetic protocol was developed and optimal reagents have been selected for obtaining guide RNA oligonucleotides for the CRISPR/Cas system using ASM-2000 automatic synthesizer in 500 nmol scale. Methods for the isolation, purification and analytical control of synthetic RNA oligonucleotides have been developed. The improved technology has been used for preparation of guide RNAs for the CRISPR Cas12a system.
In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 56, Heft 2, S. 197-205
ISSN: 2524-2342
We report herein improved version of the synthesis of hapten based on cholecalciferol and its active metabolite 25-hydroxycholecalcalferol. The methodology of obtaining high-molecular immunogenic conjugates of vitamin D3 derivatives with bovine serum albumin and horseradish peroxidase conjugates for direct ELISA was optimized. Immunisation of rabbits was carried out and polyclonal antibodies to 25-hydroxycholecalceferol were obtained and tested in an enzyme-linked immunosorbent assay. To improve the accuracy of the method, the sample preparation procedure was optimized, including the release of vitamin D3 and its active metabolites from complexes with vitamin D-binding protein.